Hsp 70 Antibody | 112-AHS0703
- Mouse monoclonal [5A5] to Hsp70
- Suitable for: IHC-P, WB, ICC/IF, IP, Flow Cyt
- Reacts with: Mouse, Rat, Dog, Human, African green monkey
- Isotype: IgG1
Reacts with: Mouse, Rat, Dog, Human, African green monkey
Recombinant fragment within Human Hsp70 aa 122-264 (N terminal). The exact sequence is proprietary.
Epitope mapping with a panel of HSP 70 deletion mutants suggests that the epitope recognized is located between amino acids 122-264 of human HSP 70, a region that has been shown to be involved in ATP binding. This is the first monoclonal antibody reported to react with: 1) The ATP binding region of HSP 70. 2) An epitope in the amino terminus of HSP 70.
- WB: HEK-293, A549, IMR-32, Jurkat, Jurkat treated with heat shock , MDCK, NIH/3T3, PC-12, COS-7, HeLa, K562, A431, U-2 OS whole cell and mouse ovary tissue lysate. IHC-P: Human prostate, tonsil and testis tissue. ICC: HeLa, NIH- 3T3, U251 MG, C6 cells. IP: HeLa cell lysates.
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